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hrp  (Bio-Rad)


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  • 99

    Structured Review

    Bio-Rad hrp
    Hrp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 5152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/l+conjugated+antibody/Goat+Anti-Mouse+IgG+(H+%2B+L)-HRP+Conjugate/pmc13091180-116-4-10
    Average 99 stars, based on 5152 article reviews
    hrp - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    FACS:

    Article Title: Association of postpartum thyroid dysfunction with antepartum hormonal and immunological changes.
    Article Snippet: .. The culture plate was placed in a humidified incubator at 37 C, gas phase 5% CO2 in air, for 4 h. Aliquots (350 l) from the stimulated and unstimulated wells were transferred into fluorescenceactivated cell sorter (FACS) tubes and the surface antigen (CD3 and CD4) was stained by adding 5 l conjugated antibody (R-phycoerythrin-Cy5 conjugated mouse antihuman CD3, DAKO Corp. Ltd., Ely, UK; fluorescein isothiocyanate-conjugated mouse antihuman CD4, Serotec Ltd., Oxford, UK) and incubated in the dark for 30 min. .. The cell suspensions were washed by centrifugation with phosphate buffer (10 mmol/liter, pH 7.4) containing sodium chloride (150 mmol/liter), sodium azide (200 mg/liter), and BSA (2 g/liter) (PBS) at 300 g for 5 min at 4 C. The supernatant was discarded and 50 l fixation and permeablization reagent A (Haralaan Sera-Lab, Loughborough, UK) was added and incubated for 15 min in the dark.

    Staining:

    Article Title: Association of postpartum thyroid dysfunction with antepartum hormonal and immunological changes.
    Article Snippet: .. The culture plate was placed in a humidified incubator at 37 C, gas phase 5% CO2 in air, for 4 h. Aliquots (350 l) from the stimulated and unstimulated wells were transferred into fluorescenceactivated cell sorter (FACS) tubes and the surface antigen (CD3 and CD4) was stained by adding 5 l conjugated antibody (R-phycoerythrin-Cy5 conjugated mouse antihuman CD3, DAKO Corp. Ltd., Ely, UK; fluorescein isothiocyanate-conjugated mouse antihuman CD4, Serotec Ltd., Oxford, UK) and incubated in the dark for 30 min. .. The cell suspensions were washed by centrifugation with phosphate buffer (10 mmol/liter, pH 7.4) containing sodium chloride (150 mmol/liter), sodium azide (200 mg/liter), and BSA (2 g/liter) (PBS) at 300 g for 5 min at 4 C. The supernatant was discarded and 50 l fixation and permeablization reagent A (Haralaan Sera-Lab, Loughborough, UK) was added and incubated for 15 min in the dark.

    Incubation:

    Article Title: Association of postpartum thyroid dysfunction with antepartum hormonal and immunological changes.
    Article Snippet: .. The culture plate was placed in a humidified incubator at 37 C, gas phase 5% CO2 in air, for 4 h. Aliquots (350 l) from the stimulated and unstimulated wells were transferred into fluorescenceactivated cell sorter (FACS) tubes and the surface antigen (CD3 and CD4) was stained by adding 5 l conjugated antibody (R-phycoerythrin-Cy5 conjugated mouse antihuman CD3, DAKO Corp. Ltd., Ely, UK; fluorescein isothiocyanate-conjugated mouse antihuman CD4, Serotec Ltd., Oxford, UK) and incubated in the dark for 30 min. .. The cell suspensions were washed by centrifugation with phosphate buffer (10 mmol/liter, pH 7.4) containing sodium chloride (150 mmol/liter), sodium azide (200 mg/liter), and BSA (2 g/liter) (PBS) at 300 g for 5 min at 4 C. The supernatant was discarded and 50 l fixation and permeablization reagent A (Haralaan Sera-Lab, Loughborough, UK) was added and incubated for 15 min in the dark.



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